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Additionally, BSE images can be more susceptible to charging artifacts in non-conductive samples, as the high-energy backscattered electrons can cause localized charging on the sample surface.
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[citation needed] Low-voltage SEM is typically conducted in an instrument with a field emission guns (FEG) which is capable of producing high primary electron brightness and small spot size even at low accelerating potentials. To prevent charging of non-conductive specimens, operating conditions must be adjusted such that the incoming beam current is equal to sum of outgoing secondary and backscattered electron currents, a condition that is most often met at accelerating voltages of 0.3–4 kV.[citation needed]
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Due to the very narrow electron beam, SEM micrographs have a large depth of field yielding a characteristic three-dimensional appearance useful for understanding the surface structure of a sample.
However, it might not be very versatile or achieve very high resolution. Hinein an SEM, as in scanning Tutorium microscopy, magnification results from the ratio of the raster on the display device and dimensions of the raster on the specimen. Assuming that the display screen has a fixed size, higher magnification results from reducing the size of the raster on the specimen, and vice versa. Magnification is therefore controlled by the current supplied to the x, Ypsilon scanning coils, or the voltage supplied to the x, y deflector plates, and not by objective lens power.
However, backscattered electron imaging also has some limitations. The resolution of BSE images is generally lower than that of SE images, check here as the backscattered electrons originate from a larger interaction volume within the sample.
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[23] The carbon dioxide is finally removed while in a supercritical check here state, so that no gas–liquid interface is present within the sample during drying.